Cy3 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1009-1
1 mg
$95.00
In stock
1009-5
5 mg
$315.00
In stock
1009-25
25 mg
$790.00
In stock
1009-100
100 mg
$1,950.00
In stock

    The maleimide derivative of Cy3 dye is the most popular tool for conjugating the dye to a thiol group on antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

    Abs/Em Maxima
    553/569 nm
    Extinction Coefficient
    150,000
    Flow Cytometry Laser Line
    532, 555 or 568 nm
    Microscopy Laser Line
    532 or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
    Molecular weight
    739.87 (protonated)
    CAS
    n/a
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    The maleimide derivative of Cy3 dye is the most popular tool for conjugating the dye to a thiol group on antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

    Cy3 Amine (also sold under Sulfo-Cyanine3 Amine) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

    This sulfonated dye is also known as sulfo-Cyanine3 Maleimide.

    Cy®Dye is a trademark of GE Healthcare.

    Cy3

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