Cy3 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1009-1
1 mg
$95.00
In stock
1009-5
5 mg
$290.00
In stock
1009-25
25 mg
$595.00
In stock
1009-100
100 mg
$1,450.00
In stock

The maleimide derivative of Cy3 dye is the most popular tool for conjugating the dye to a thiol group on antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

Abs/Em Maxima
553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight
739.87 (protonated)
CAS
n/a
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

The maleimide derivative of Cy3 dye is the most popular tool for conjugating the dye to a thiol group on antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

Cy3 Amine (also sold under Sulfo-Cyanine3 Amine) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

This sulfonated dye is also known as sulfo-Cyanine3 Maleimide.

Cy®Dye is a trademark of GE Healthcare.

Cy3

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