Cy®3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy3 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
Cy®3 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
This is sulfonated dye is also known as sulfo-Cyanine3. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||550/570 nm|
|Flow cytometry laser line||532 555 or 568 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549|
|Solubility||Water, DMSO, DMF|
|Shipping conditions||Ambient temperature|