Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
Cy®3 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy3 is a water-soluble, bright fluorescent label that can be used in place of Alexa Fluor 555®, DyLight 549® and other dyes excitable at a wavelength of 532 or 555 nm.
For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.
This sulfonated dye is also known as sulfo-Cyanine3 Azide. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||550/570 nm|
|Flow cytometry laser line||532 or 555 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|
|Cy3 Picolyl Azide||A water-soluble probe with superior kinetics in copper-catalyzed click reactions.|
|Click Chemistry Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|