Cy3 Azide
Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.
Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.
This is sulfonated dye is also known as sulfo-Cyanine3.
Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
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A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions.
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Alkyne-containing, noncanonical amino acid.
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