Cy3 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
AZ119-1
1 mg
$95.00
In stock
AZ119-5
5 mg
$295.00
In stock
AZ119-25
25 mg
$690.00
In stock
AZ119-100
100 mg
$1,850.00
In stock

Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Abs/Em Maxima
553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight
806.96 (protonated)
CAS
1782950-79-1
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.

This is sulfonated dye is also known as sulfo-Cyanine3.

Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

Cy3

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