Cy3 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
TA117-1
1 mg
$115.00
In stock
TA117-5
5 mg
$369.00
In stock
TA117-25
25 mg
$840.00
In stock
TA117-100
100 mg
$2,450.00
In stock

    Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

    Abs/Em Maxima
    553/569 nm
    Extinction Coefficient
    150,000
    Flow Cytometry Laser Line
    532, 555 or 568 nm
    Microscopy Laser Line
    532 or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
    Molecular weight
    761.92 (protonated)
    CAS
    n/a
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C
    Shipping conditions
    Ambient temperature

    Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

    Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.

    This is sulfonated dye is also known as sulfo-Cyanine3.

    Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    Cy3

    1. Banerjee, C., et al. (2022). Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J Vis Exp., 186. [PubMed]
    2. Schamber, M. R., et al. (2022). Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2. Elife, 11:e78982. [PubMed]
    3. Schamber, M. R., et al. (2022). Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nat Commun., 13 (1), 2194. [PubMed]
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