Cy3 Alkyne
Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.
Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.
This is sulfonated dye is also known as sulfo-Cyanine3.
Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
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Excitation maximum: 555 nm
Emission maximum: 572 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 648 nm Emission maximum: 671 nm