Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
The orange-fluorescent Cy®3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC). Cy®3 is a water-soluble, bright fluorescent label that can be used in place of Alexa Fluor 555®, DyLight 549® and other dyes excitable at a wavelength of 532 or 555 nm.
This sulfonated dye is also known as sulfo-Cyanine3 Alkyne. Cy®Dye is a trademark of GE Healthcare.
|Abs/Em Maxima||550/570 nm|
|Flow cytometry laser line||532 or 555 nm|
|Microscopy laser line||532 or 555 nm|
|Spectrally similar dyes||Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549|
|Solubility||Water, DMSO, DMF, MeOH|
|Shipping conditions||Ambient temperature|