Click-&-Go Protein Enrichment Kit for Capture of Azido-tagged Proteins
Click-&-GoTM Protein Enrichment Kit is an efficient, biotin-streptavidin free tool for capture of azido-modified proteins on an alkyne-agarose resin. The azide modification can occur via metabolic feeding, enzymatic addition, or chemical modification. The azido-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the alkyne-agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.
The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.
The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.
The Click-&-GoTM Protein Enrichment Kit can be used as direct replacement of Click-iT® Protein Enrichment Kit