Click-&-Go Plus EdU PB Flow Cytometry Assay Kit, 50 assays
Click-&-Go Plus EdU is the next step in improving the biocompatibility and sensitivity of traditional EdU-based proliferation assays. The copper concentrations typically used in traditional click chemistry reactions can affect fluorophores such as green fluorescent protein, mCherry, and R-phycoerythrin which can result in loss of fluorescence signal. The advantage of the Click-&-Go Plus EdU assay is that it employs the newest generation of copper-chelating azides, Azide Plus probes. These Azide Plus probes form a strong copper-azide complex, and thus dramatically raise the copper concentration at the reaction site without the need to maintain the overall copper concertation at a high level. As a result, much lower copper concentrations and copper protectants can be used without losing the efficiency of the copper-catalyzed detection reaction. This makes the Click-&-Go Plus EdU assay compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry (Table 1). The Click-&-Go Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers.
Table 1. Compatibility of Click-&-Go Plus assay with detection reagents.
|R-phycoerythrin (R-PE) and R-PE based tandems (e.g., Alexa Fluor™ 610-RPE)||Compatible, except Alexa Fluor™ 594 with R-PE-Cy®7 tandems|
|PerCP, allophycocyanin (APC) and APC-based tandems (e.g. Alexa Fluor™ 680-APC)||Compatible|
|Fluorescent proteins (e.g. GFP, mCherry)||Compatible|
|Nanocrystals||Nanocrystals Not compatible, should be used after EdU detection.|
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781
Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.