Click-&-Go Plus EdU PB
Flow Cytometry Assay Kit, 50 assays
Click-&-Go Plus EdU is the next step in improving the biocompatibility and sensitivity of traditional EdU-based proliferation assays. The copper concentrations typically used in traditional click chemistry reactions can affect fluorophores such as green fluorescent protein, mCherry, and R-phycoerythrin which can result in loss of fluorescence signal. The advantage of the Click-&-Go Plus EdU assay is that it employs the newest generation of copper-chelating azides, Azide Plus probes. These Azide Plus probes form a strong copper-azide complex, and thus dramatically raise the copper concentration at the reaction site without the need to maintain the overall copper concertation at a high level. As a result, much lower copper concentrations and copper protectants can be used without losing the efficiency of the copper-catalyzed detection reaction. This makes the Click-&-Go Plus EdU assay compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry (Table 1). The Click-&-Go Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers.
Table 1. Compatibility of Click-&-Go Plus assay with detection reagents.
|R-phycoerythrin (R-PE) and R-PE based tandems (e.g., Alexa Fluor™ 610-RPE)||Compatible, except Alexa Fluor™ 594 with R-PE-Cy®7 tandems|
|PerCP, allophycocyanin (APC) and APC-based tandems (e.g. Alexa Fluor™ 680-APC)||Compatible|
|Fluorescent proteins (e.g. GFP, mCherry)||Compatible|
|Nanocrystals||Nanocrystals Not compatible, should be used after EdU detection.|
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