Click-&-Go EdU 647 Cell Proliferation Assay Kit

Click-&-Go Plus EdU Flow Cytometry Assay Kits
Catalog # 1329


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The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA. Traditional cell proliferation assays utilizes radioactive nucleosides, i.e., 3H-thymidine or antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). Both methods exhibit several limitations, including troublesome handling of radioactive materials and DNA denaturation step by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules for anti-BrdU antibody staining. Click-&-Go EdU 647 Cell Proliferation Assay for imaging a superior alternative to traditional time consuming and difficult to perform consistently cell proliferation assays. Click-&-Go EdU 647 Cell Proliferation Assay takes advantage of EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog of thymidine terminal alkyne group that is efficiently incorporated into DNA during active DNA synthesis. In the next step, EdU-labeled newly synthesized DNA is imaged through a click reaction with best-in-class far-red fluorescent Azide 647.

Product Size
1 kit
Azide 647
Abs/Em Maxima
648/671 nm
Number of Reactions
Storage Condition
Shipping Condition
Ambient temperature


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5-Ethynyl Uridine (5-EU)

An alkyne-containing uridine analogue.

EdU (5-Ethynyl-2′-deoxyuridine)

An alkyne-containing thymidine analogue.


A probe for DNA synthesis that has minimal impact on cellular proliferation.