Click-&-Go EdU 488
Flow Cytometry Assay Kit, 50 assays
The Click-&-Go EdU 488 Flow Cytometry Assay Kit is a superior alternative to the traditional cell proliferation assays that use radioactive nucleosides, i.e., 3H-thymidine or antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog of thymidine with a terminal alkyne group that is efficiently incorporated into DNA during active DNA synthesis. In the next step, newly synthesized EdU-labeled DNA is fluorescently labeled through a click reaction with the best-in-class green fluorescent Azide 488 (Alexa Fluor® 488 Azide equivalent). Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population. Sufficient amounts of reagents are provided for 50 assays based on the following protocol.
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