Click-&-Go® EdU 488
Flow Cytometry Assay Kit, 50 assays • Discontinued
$249.00
Out of stock
The Click-&-Go® EdU 488 Flow Cytometry Assay Kit is a superior alternative to the traditional cell proliferation assays that use radioactive nucleosides, i.e., 3H-thymidine or antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog of thymidine with a terminal alkyne group that is efficiently incorporated into DNA during active DNA synthesis. In the next step, newly synthesized EdU-labeled DNA is fluorescently labeled through a click reaction with the best-in-class green fluorescent Azide 488 (Alexa Fluor® 488 Azide equivalent). Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population. Sufficient amounts of reagents are provided for 50 assays based on the following protocol.
Alexa Fluor® is a registered trademarks of Thermo Fisher Scientific.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Alkyne-containing, noncanonical amino acid.
An alkyne-containing thymidine analogue.
An alkyne-containing uridine analogue.