Click Chemistry Reaction Buffer Kit
Click Chemistry Reaction Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 efficient copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.The kit utilizes THPTA as a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.
Sufficient reagents are provided for 25 labeling reactions containing 50 uL protein solution (1-5 mg/mL).
|Product Size||1 kit|
|Number of Reactions||25|
|Shipping Condition||Ambient temperature|
|5-TAMRA Azide||Azide-activated single isomer 5-TAMRA dye is exact replacement of Invitrogen’s (Thermo Fisher Scientific) Tetramethylrhodamine Azide, 5-isomer (Catalog number: T10182).|
|5-TAMRA Picolyl Azide||A single isomer advanced probe with superior kinetics in copper-catalyzed click reactions.|
|Biotin-PEG3-Azide||An azide-activated biotinylation reagent with a hydrophilic spacer arm.|
|OG 488 Azide||A generic version of Oregon Green® 488 Azide.|