Carboxyrhodamine 110 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
A127-1
1 mg
$115.00
In stock
A127-5
5 mg
$395.00
In stock
A127-25
25 mg
$1,095.00
In stock

Carboxyrhodamine 110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine 110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Maxima
501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes
Fluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight
880.96
CAS
N/A
Solubility
DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Carboxyrhodamine110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Carboxyrhodamine110, a non-sulfonated version of Alexa Fluor® 488, is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. Its absorption/emission spectra is a similar to those of DyLight® 488, Alexa Fluor® 488.

Carboxyrhodamine110 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.

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