Carboxyrhodamine 110 Azide
5(6)-Carboxyrhodamine 110 (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright and exceedingly photostable and pH-insensitive from pH 4 to pH 10 green-fluorescent probe. Unlike Alexa Fluor® 488, 5(6)-Carboxyrhodamine 110 is stable at highly alkaline conditions. This probe can be used with the 488 nm line of argon-ion laser and standard FITS filter set.
5(6)-Carboxyrhodamine 110 Azide is green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
|Abs/Em Maxima||501/523 nm|
|Flow cytometry laser line||488 nm|
|Microscopy laser line||488 nm|
|Spectrally similar dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF™ 488A dyes, DyLight™488,|
|Shipping conditions||Ambient temperature|
|MB 488 Azide||A bright, exceedingly photostable and pH-insensitive green fluorescence-emitting probe.|
|MB 488 Picolyl Azide||A bright and photostable green fluorescence-emitting probe with superior kinetics in copper-catalyzed click reactions.|
|FAM Picolyl Azide||A green fluorescence-emitting probe with superior kinetics in copper-catalyzed click reactions.|
|Click Chemistry Reaction Buffer Kit||All-inclusive, click reaction enabling kit for protein labeling.|
|THPTA||A water-soluble ligand for copper-catalyzed azide-alkyne cycloadditions (CuAAC).|