BTTES is a newest generation, water-soluble accelerating ligand for CuAAC that provides much greater rate enhancement compared to previous generation ligands (e.g. THPTA or TBTA). More importantly, it minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering Cu loading in the catalyst formulation.
1. BSoriano del Amo, D., et al. (2010). Biocompatible copper(I) catalysts for in vivo imaging of glycans J. Am. Chem. Soc.,. 132(47): 16893–9. [PubMed]
An azide-activated biotinylation reagent.
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