Biotin Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1167-5
5 mg
$129.00
In stock
1167-25
25 mg
$295.00
In stock
1167-100
100 mg
$895.00
In stock

Biotin Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boosted the efficiency of the CuAAC reaction resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides was reported (see Selected References).

Learn more about picolyl azide reagents.

Molecular weight
622.74
Chemical composition
C27H42N8O7S
CAS
N/A
Solubility
DMSO, DMF
Purity
>95% (HPLC)
Appearance
Off-white to slightly orange amorphous solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

Biotin Picolyl Azide is an advanced azide probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

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