Biotin-PEG4-NHS Ester

Catalog # Pkg Size Price(USD) Quantity
B103-2 4 x 2 mg $95.00
B103-25 25 mg $95.00
B103-100 100 mg $195.00
B103-1G 1000 mg $695.00

Biotin-PEG4-NHS Ester reacts specifically and efficiently with primary amines (e.g., a side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

 

Protein Labeling Calculator

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  • Volume of DMSO required to make a stock solution (µL)

  • Volume of protein to be labeled (µL)

  • Volume of biotin stock solution to add (µL)

  • Percent DMSO in Reaction Mixture (%)

Caution - Volume of DSMO solvent added to protein must always be <5% total volume of reaction