Biotin-PEG4-NHS Ester

B103-25

25 mg

$109.00

In stock

B103-100

100 mg

$185.00

In stock

B103-1G

1000 mg

$620.00

In stock

Biotin-PEG4-NHS Ester reacts specifically and efficiently with primary amines (e.g., a side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Molecular weight

588.67

Chemical composition

C25H40N4O10S

CAS

459426-22-3

Solubility

DMSO, DMF, DCM, THF, Chloroform

Purity

>95%

Appearance

White to grey amorphous solid

Storage conditions

-20°C. Desiccate

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) *

* Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Search specific Certificate of Analysis (CoA) by LOT number: