Biotin-PEG4-Alkyne is non-cleavable, alkyne-activated biotinylation reagent that can be reacted with azides via a copper-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. The hydrophilic polyethylene glycol (PEG) spacer provides a long and flexible connection that minimizes steric hindrance involved with binding to avidin molecules.
- Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
- Bernardim, B., et al. (2020). Precise Installation of Diazo-Tagged Side-Chains on Proteins to Enable In Vitro and In-Cell Site-Specific Labeling Bioconjugate Chem., 31(6), 1604-10. [PubMed]