Biotin-PEG4-Alkyne reacts with azides via a copper-catalyzed click reaction to produce a stable triazole linkage. The hydrophilic polyethylene glycol (PEG) spacer arm imparts solubility in aqueous buffers and provides a long and flexible connection that minimizes steric hindrance involved with binding to avidin molecules. Biotin can be subsequently used for detection or affinity purification.
|Solubility||DMSO, DMF, MeOH|
|Appearance||White to grey amorphous solid|
|Shipping conditions||Ambient temperature|