Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction to produce a stable triazole linkage. It also reacts with cyclooctynes via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This reaction is bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment. This reagent is also know as Biotin-Azide.
For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.
|Appearance||White to grey amorphous solid|
|Shipping conditions||Ambient temperature|
|Biotin Picolyl Azide||Biotinylation reagent with superior kinetics in copper-catalyzed click reactions.|