Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.
- Hu, Y., Glazier, R., et al. (2021). DNA-based microparticle tension sensors (µTS) for measuring cell mechanics in non-planar geometries and for high-throughput quantification. Angew Chem Int Ed Engl., Online ahead of print. [PubMed]
- Bazrafshan, A. et al. (2021). DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. ACS Publications, Online ahead of print. [PubMed]
- Bazrafshan, A., et al. (2020). Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds. Angew. Chem. Int. Ed., 59(24), 9514-21. [PubMed]