AZDye 680R Tetrazine
AZDye 680R is a bright and photostable near IR dye that emits fluorescence at about 700 nm in the borderline spectral region between far-red and near-IR. AZDye 680R dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 680R makes this dye an ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability.
Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.
AZDye 680R is a bright and photostable near IR dye that emits fluorescence at about 700 nm in the borderline spectral region between far-red and near-IR. AZDye 680R dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 680R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AZDye 680R makes this dye an ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability.
AZDye 660R dye spectrally is almost identical to CF® 680R Dye and can be used a less expensive alternative to these dyes.
CF® Dye is a registered trademark of Biotium.
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 648 nm Emission maximum: 671 nm
Excitation maximum: 590 nm Emission maximum: 617 nm