AZDye 680 Tyramide

Unit Size
Price (USD)
150 slides
In stock
1500 slides
In stock

    NIR fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

    AZDye™ 680 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

    Abs/Em Maxima
    678/701 nm
    Extinction Coefficient
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye
    Molecular weight
    947.57 (protonated)
    Water, DMSO, DMF
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature


    Related Products
    Click-&-Go® Click Chemistry Reaction Buffer Kit

    A general purpose click chemistry reaction buffer kit.

    labeling kit$170.00
    O-propargyl-puromycine (OPP)

    An alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media.

    L-Homopropargylglycine (HPG)

    Alkyne-containing, noncanonical amino acid.