AFDye 680 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1515-1
1 mg
$139.00
In stock
1515-5
5 mg
$435.00
In stock
1515-25
25 mg
$1,350.00
In stock
1515-100
100 mg
$2,750.00
In stock

    AFDye 680 (Alexa Fluor ® 680 equivalent) is a bright and photostable near-IR dye that is spectrally identical to Alexa Fluor® 680 and IRDye® 680RD. AFDye 680 is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. AFDye 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

    The NHS ester (succinimidyl ester) of AFDye 680 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

    Abs/Em Maxima
    678/701 nm
    Extinction Coefficient
    185,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye
    Molecular weight
    924.21 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >90% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    Cy5.5