AFDye 660R Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1503-1
1 mg
$149.00
In stock
1503-5
5 mg
$435.00
In stock
1503-25
25 mg
$1,395.00
In stock

AFDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AFDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AFDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

Abs/Em Maxima
665/690 nm
Extinction Coefficient
92,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar Dyes
Alexa Fluor® 660, CF® 660R
Molecular weight
946.08 (protonated)
CAS
n/a
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye 660R Picolyl Azide is a bright and very photostable probe routinely used for imaging of alkyne-containing biomolecules. MB 660R Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AFDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AFDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AFDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

AFDye660

AFDye 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.

Cy5

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

Related Products
AFDye 647 DBCO

Excitation maximum: 648 nm
Emission maximum: 671 nm

AFDye 488 DBCO

Excitation maximum: 494 nm
Emission maximum: 517 nm

AFDye 594 DBCO

Excitation maximum: 590 nm
Emission maximum: 617 nm