AFDye 660R Picolyl Azide
AFDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AFDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AFDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.
AFDye 660R Picolyl Azide is a bright and very photostable probe routinely used for imaging of alkyne-containing biomolecules. MB 660R Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AFDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AFDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AFDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AFDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.
AFDye 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.
1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
Excitation maximum: 648 nm
Emission maximum: 671 nm
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 590 nm
Emission maximum: 617 nm
