AFDye 647 Tyramide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1543-1
150 slides
$149.00
In stock
1543-2
1500 slides
$875.00
In stock

    Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

    AFDye™ 647 Tyramide (Alexa Fluor® 647 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

    Alexa Fluor® 647 is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    648/671 nm
    Extinction Coefficient
    270,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649
    Molecular weight
    992.20 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye647