AZDye 647 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1300-1
1 mg
$195.00
In stock
1300-5
5 mg
$595.00
In stock
1300-25
25 mg
$1,895.00
In stock

    AZDye™ 647 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Learn more about picolyl azide reagents.
    Abs/Em Maxima
    648/671 nm
    Extinction Coefficient
    270,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649
    Molecular weight
    1061.21 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 647 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    AZDye™ 647 Picolyl Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    AZDye647

    1. Ratnayeke, N., et al. (2022). CDC7-independent G1/S transition revealed by targeted protein degradation. Nature., 605 (7909), 357-365. [PubMed]
    2. Ratnayeke, N., et al. (2021). Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. bioRxiv, e-print. [bioRxiv]
    3. Köberlin, M. S., et al. (2021). LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol., 220 (8), e202009147. [PubMed]
    4. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
    5. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    6. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]