AFDye 647 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1344-1
1 mg
$139.00
In stock
1344-5
5 mg
$455.00
In stock
1344-25
25 mg
$1,395.00
In stock
1344-100
100 mg
$2,695.00
In stock

    AFDye™ 647 NHS Ester is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    648/671 nm
    Extinction Coefficient
    270,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649, Cy5 Dye
    Molecular weight
    970.10 (protonated) 1273.68 (triethylammonium salt)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 647 is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

    The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AFDye™ 647 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 647 conjugates are often a probe of choice for detection of low abundance targets.

    AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes, and can be used as a less expensive alternative to Alexa Fluor® 647 NHS Ester.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    AFDye647

    1. Lehmann, M., et al. (2020). Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series. bioRxiv, 10, 1101. [bioRxiv]