AFDye 647 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1526-1
1 mg
$139.00
In stock
1526-5
5 mg
$445.00
In stock
1526-25
25 mg
$1,395.00
In stock
1526-100
100 mg
$2,795.00
In stock

    AFDye™ 647 Maleimide (Alexa Fluor® 647 Maleimide analog) is a far-red-fluorescent sulfhydryl-reactive dye. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    648/671 nm
    Extinction Coefficient
    270,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649, Cy5 Dye
    Molecular weight
    994.25 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 647 is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. This dye is water soluble and pH-insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

    Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

    AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes, and can be used as a less expensive alternative to Alexa Fluor® 647 C2 Maleimide.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    AFDye647

    1. Lehmann, M., et al. (2020). Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series. bioRxiv, 10, 1101. [bioRxiv]