AFDye 647 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1302-1
1 mg
$149.00
In stock
1302-5
5 mg
$485.00
In stock
1302-25
25 mg
$1,495.00
In stock

    AFDye™ 647 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

    Alexa Fluor® 647 is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    648/671 nm
    Extinction Coefficient
    270,000
    Flow Cytometry Laser Line
    633 nm or 647 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 647, CF® 647, DyLight® 649
    Molecular weight
    1131.36
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 647 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

    AFDye™ 647 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AFDye™ 647 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 647 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AFDye™ 647 DBCO can be used a less expensive alternative to Alexa Fluor® 647 sDIBO.

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    AFDye647