AFDye 647 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1301-1
1 mg
$139.00
In stock
1301-5
5 mg
$435.00
In stock
1301-25
25 mg
$1,395.00
In stock

AFDye™ 647 Alkyne is a water-soluble, bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines and visualized with Cy5 filter sets. AFDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 647 DBCO or Cy5 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of ThermoFisher Scientific.

Abs/Em Maxima
648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar Dyes
Alexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight
910.10 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 647 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines, and visualized with Cy5 filter sets.

AFDye™ 647 is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AFDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.

For application where the presence of copper is not acceptable, please consider our AFDye™ 647 DBCO or Cy5 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye647