AZDye 633 Picolyl Azide
AZDye™ 633 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
AZDye™ 633 Azide is a bright and photostable far-red fluorescent probe with excitation ideally suited to the 633 nm or 635 nm laser excitation source. AZDye™ 633 Azide is water-soluble, pH-insensitive from pH 4 to pH 10. AZDye™ 633 spectrally is almost identical to Alexa Fluor® 633, DyLight® 633 or CF® 633 Dye. Combination of superior brightness and very low autofluorescence background signal in most biological samples in far-red spectral region allows for very sensitive detection of alkyne-labeled biomolecules.
Alexa Fluor® 633 and DyLight® 633 are registered trademarks of Thermo Fisher Scientific.Learn more about picolyl azide reagents.
- Morral, C., et al. (2020). Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro. STAR Protocols, Volume 1, 2 [ScienceDirect]
- Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry, 10, 1093. [Oxford Academic]
- Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
- Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
- Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
Excitation maximum: 753 nm
Emission maximum: 775 nm
Excitation maximum: 678 nm
Emission maximum: 701 nm
Excitation maximum: 648 nm Emission maximum: 671 nm