AFDye 594 Tyramide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1542-1
1 mg
$149.00
In stock
1542-2
5 mg
$875.00
In stock

    Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

    AFDye™ 594 Tyramide (Alexa Fluor® 594 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

    Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    590/617 nm
    Extinction Coefficient
    88,000
    Flow Cytometry Laser Line
    561 nm or 594 nm
    Microscopy Laser Line
    594 nm
    Spectrally Similar Dyes
    Alexa Fluor® 594, CF® 594, DyLight® 594
    Molecular weight
    841.95 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Dark blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye594

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    Excitation maximum: 648 nm
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