AZDye 594 TFP Ester
AZDye™ 594 TFP Ester is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AZDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AZDye™ 594 TFP Ester is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AZDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
A2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.
AZDye™ 594 is structurally identical to Alexa Fluor® 594.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Excitation maximum: 648 nm Emission maximum: 671 nm
Excitation maximum: 590 nm Emission maximum: 617 nm