AFDye 594 TFP Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1433-100μg
3 x 100 μg
$139.00
In stock
1433-1
1 mg
$139.00
In stock
1433-5
25 mg
$435.00
In stock
1433-25
25 mg
$1,495.00
In stock

AFDye™ 594 TFP Ester (Alexa Fluor 594 TFP Ester equivalent) is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Maxima
590/617 nm
Extinction Coefficient
92,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight
870.33 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>80% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 594 TFP Ester is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

A2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.

AFDye™ 594 is structurally identical to Alexa Fluor® 594.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye594

third content

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AFDye 647 DBCO

Excitation maximum: 648 nm
Emission maximum: 671 nm

AFDye 594 DBCO

Excitation maximum: 590 nm
Emission maximum: 617 nm