AFDye 594 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1343-1
1 mg
$139.00
In stock
1343-5
5 mg
$395.00
In stock
1343-25
25 mg
$1,395.00
In stock
1343-100
100 mg
$2,695.00
In stock

AFDye™ 594 NHS Ester (Alexa Fluor 594 NHS Ester equivalent) is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Maxima
590/617 nm
Extinction Coefficient
90,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight
819.85
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>80% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 594 NHS Ester (Alexa Fluor 594 NHS Ester equivalent) is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 594 NHS Ester is structurally identical to Alexa Fluor® 594 NHS Ester and spectrally is almost identical to CF® 594, DyLight® 594, or any other fluorescent dyes based on sulfonated rhodamine core.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dyes is a registered trademark of Biotium Inc.

AFDye594

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AFDye 647 DBCO

Excitation maximum: 648 nm
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AFDye 594 DBCO

Excitation maximum: 590 nm
Emission maximum: 617 nm