AFDye 594 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1525-1
1 mg
$139.00
In stock
1525-5
5 mg
$445.00
In stock
1525-25
25 mg
$1,395.00
In stock
1525-100
100 mg
$2,895.00
In stock

    AFDye 594 Maleimide (Alexa Fluor® 594 C5 Maleimide equivalent) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

    Abs/Em Maxima
    590/617 nm
    Extinction Coefficient
    90,000
    Flow Cytometry Laser Line
    561 nm or 594 nm
    Microscopy Laser Line
    594 nm
    Spectrally Similar Dyes
    Alexa Fluor® 594, CF® 594, DyLight® 594
    Molecular weight
    885.98
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Dark blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 594 Maleimide (Alexa Fluor® 594 C5 Maleimide equivalent) is a sulfhydryl-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary thiols (Cys), thiol-modified oligonucleotides, and other thiol-containing biomolecules. AFDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

    AFDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

    Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

    Alexa Fluor® is registered trademarks of Thermo Fisher Scientific.

    AFDye594

    Related Products
    AFDye 647 DBCO

    Excitation maximum: 648 nm
    Emission maximum: 671 nm

    AFDye 594 DBCO

    Excitation maximum: 590 nm
    Emission maximum: 617 nm