AZDye 594 Biotin

Catalog#
Unit Size
Price (USD)
Availability
Qty
1396-1
1 mg
$179.00
In stock
1396-5
5 mg
$595.00
In stock

    AZDye 594 Biotin (Alexa Fluor® 594 Biotin equivalent) can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye 594 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

    Abs/Em Maxima
    590/617 nm
    Extinction Coefficient
    88,000
    Flow Cytometry Laser Line
    532 or 594 nm
    Microscopy Laser Line
    594 nm
    Spectrally Similar Dyes
    Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594
    Molecular weight
    1123.32
    CAS
    N/A
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >95% (HPLC)
    Appearance
    Blue solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    MB594

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