AFDye 594 Biotin

Catalog#
Unit Size
Price (USD)
Availability
Qty
1396-1
1 mg
$149.00
In stock
1396-5
5 mg
$535.00
In stock

AFDye 594 Biotin (Alexa Fluor® 594 Biotin equivalent) can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AFDye 594 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Abs/Em Maxima
601/623 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594
Molecular weight
1123.32
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

MB594

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