AFDye 594 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1295-1
1 mg
$139.00
In stock
1295-5
5 mg
$395.00
In stock
1295-25
25 mg
$1,295.00
In stock

AFDye™ 594 Azide (Alexa Fluor® 594 equivalent) is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. AFDye™ 594 is well suited for the 561 nm and 594 nm laser lines of the Ar-Kr mixed-gas laser.

Alexa Fluor® 594 is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight
864.97 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 594 Azide is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 594 (Alexa Fluor® 594 equivalent) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AFDye™ 594 dye structurally is identical to Alexa Fluor® 594 sold by Thermo Fisher Scientific.

Alexa Fluor® 594 is a registered trademark of Thermo Fisher Scientific.

AFDye594

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