AZDye 594 Azide
AZDye™ 594 Azide is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. AZDye™ 594 is well suited for the 561 nm and 594 nm laser lines of the Ar-Kr mixed-gas laser.
AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
Excitation maximum: 648 nm
Emission maximum: 671 nm
An alkyne-containing uridine analogue.
An alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media.