AFDye 594 Alkyne

AFDye™ 594 Alkyne (Alexa Fluor 594 Alkyne equivalent) is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AFDye™ 594 dye structurally is identical to Alexa Fluor® 594 sold by Thermo Fisher Scientific.

For application where the presence of copper is not acceptable, please consider our AFDye™ 594 DBCO or MB 594 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® 594 is a registered trademark of Thermo Fisher Scientific.