AFDye 594 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1297-1
1 mg
$139.00
In stock
1297-5
5 mg
$395.00
In stock
1297-25
25 mg
$1,295.00
In stock

AFDye™ 594 Alkyne (Alexa Fluor 594 Alkyne equivalent) is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 594 DBCO or MB 594 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of ThermoFisher Scientific.

Abs/Em Maxima
590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes
Alexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight
758.18 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 594 Alkyne (Alexa Fluor 594 Alkyne equivalent) is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AFDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AFDye™ 594 dye structurally is identical to Alexa Fluor® 594 sold by Thermo Fisher Scientific.

For application where the presence of copper is not acceptable, please consider our AFDye™ 594 DBCO or MB 594 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® 594 is a registered trademark of Thermo Fisher Scientific.

AFDye594

Related Products
AFDye 647 DBCO

Excitation maximum: 648 nm
Emission maximum: 671 nm