AZDye 594 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1297-1
1 mg
$149.00
In stock
1297-5
5 mg
$509.00
In stock
1297-25
25 mg
$1,695.00
In stock

    AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    A probe for copper-less azide detection (AZDye™ 594 DBCO) is also available for application where the presence of copper is not acceptable.

    Abs/Em Maxima
    590/617 nm
    Extinction Coefficient
    88,000
    Flow Cytometry Laser Line
    561 nm or 594 nm
    Microscopy Laser Line
    594 nm
    Spectrally Similar Dyes
    Alexa Fluor® 594, CF® 594, DyLight® 594
    Molecular weight
    758.18 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.

    For application where the presence of copper is not acceptable, please consider our AZDye™ 594 DBCO probe for copper-less detection of azide-modified molecules.

    Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

    AZDye594

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    AZDye 647 DBCO

    Excitation maximum: 648 nm Emission maximum: 671 nm