AFDye 568 TFP Ester
AFDye™ 568 TFP Ester (Alexa Fluor® 568 TFP Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AFDye™ 568 TFP Ester (Alexa Fluor® 568 TFP Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
AFDye™ 568 is a water-soluble, photostable, and bright orange dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 568 dye ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. AF™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.
AFDye™ 568 is structurally identical to Alexa Fluor® 568.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dyes is a registered trademark of Biotium Inc.
Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm
