AFDye 568 TFP Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1432-100μg
3 x 100 μg
$139.00
In stock
1432-1
1 mg
$139.00
In stock
1432-5
5 mg
$435.00
In stock
1432-25
25 mg
$1,495.00
In stock

AFDye™ 568 TFP Ester (Alexa Fluor® 568 TFP Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Maxima
578/602 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Spectrally Similar Dyes
Alexa Fluor® 568, CF® 568
Molecular weight
791.80 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>80% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 568 TFP Ester (Alexa Fluor® 568 TFP Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AFDye™ 568 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AFDye™ 568 is a water-soluble, photostable, and bright orange dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 568 dye ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. AF™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.

AFDye™ 568 is structurally identical to Alexa Fluor® 568.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dyes is a registered trademark of Biotium Inc.

AFDye568

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label
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