AFDye 568 DBCO
AFDye™ 568 DBCO (Alexa Fluor® 568 DBCO equivalent) reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 568 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
AFDye™ 568 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AFDye™ 568 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AFDye 568™ (Alexa Fluor® 568 equivalent) is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AFDye™ 568 dye structurally is identical to Alexa Fluor® 568.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm