AFDye 568 Alkyne
AFDye™ 568 Alkyne (Alexa Fluor® 568 Azide equivalent) is a water-soluble, bright, red-fluorescent dye with excitation ideally suited for the 568 nm laser lines. AFDye™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
A probe for copper-less azide detection (AFDye™ 568 DBCO) is also available for application where the presence of copper is not acceptable.
Alexa Fluor® is a registered trademark of ThermoFisher Scientific.
AFDye™ 568 Alkyne is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AFDye™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AFDye 568™ (Alexa Fluor® 568 equivalent) is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AFDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye.
For application where the presence of copper is not acceptable, please consider our AFDye™ 568 DBCO probes for copper-less detection of azide-modified molecules.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.