AFDye 555 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1523-1
1 mg
$139.00
In stock
1523-5
5 mg
$445.00
In stock
1523-25
25 mg
$1,395.00
In stock
1523-100
100 mg
$2,795.00
In stock

    AFDye™ 555 Maleimide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AFDye 555 label at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 555 conjugates ideally suited for detection of low abundance targets.

    Abs/Em Maxima
    555/572 nm
    Extinction Coefficient
    155,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    532 nm or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
    Molecular weight
    968.23 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 555 Maleimide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AFDye 555 label at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye™ 555 conjugates ideally suited for detection of low abundance targets.

    Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

    AFDye™ 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

    AFDye555

    1. Lehmann, M., et al. (2020). Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series. bioRxiv, 10, 1101. [bioRxiv]
    2. Ingham, E. S., et al. (2020). Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation. Nat Commun, 11, 2102. [Nature Communications]
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