AFDye 555 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1290-1
1 mg
$149.00
In stock
1290-5
5 mg
$495.00
In stock
1290-25
25 mg
$1,495.00
In stock

    AFDye™ 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    555/572 nm
    Extinction Coefficient
    155,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    633 nm or 647 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
    Molecular weight
    1105.32 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

    AFDye™ 555 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AFDye™ 555 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 555 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    AFDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AFDye™ 555 DBCO can be used a less expensive alternative to Alexa Fluor® 555 sDIBO.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

    AFDye555

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