AFDye 555 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1290-1
1 mg
$139.00
In stock
1290-5
5 mg
$435.00
In stock
1290-25
25 mg
$1,395.00
In stock

AFDye™ 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar Dyes
Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight
1105.32 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AFDye™ 555 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AFDye™ 555 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 555 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AFDye™ 555 DBCO can be used a less expensive alternative to Alexa Fluor® 555 sDIBO.

Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

AFDye555

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