AFDye 555 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1289-1
1 mg
$139.00
In stock
1289-5
5 mg
$465.00
In stock
1289-25
25 mg
$1,395.00
In stock

    AFDye™ 555 Alkyne is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    A probe for copper-less azide detection (AFDye™ 555 DBCO or Cy3 DBCO) is also available for application where the presence of copper is not acceptable.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    555/572 nm
    Extinction Coefficient
    155,000
    Flow Cytometry Laser Line
    532 nm or 555 nm
    Microscopy Laser Line
    532 nm or 555 nm
    Spectrally Similar Dyes
    Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
    Molecular weight
    883.06 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 555 Alkyne is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

    AFDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AFDye™ 555 Alkyne can be used a less expensive alternative to Alexa Fluor® 555 Alkyne.

    For application where the presence of copper is not acceptable, please consider our AFDye™ 555 DBCO or Cy3 DBCO probes for copper-less detection of azide-modified molecules.

    Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AFDye555

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