AFDye 555 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1289-1
1 mg
$139.00
In stock
1289-5
5 mg
$435.00
In stock
1289-25
25 mg
$1,395.00
In stock

AFDye™ 555 Alkyne is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 555 DBCO or Cy3 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight
883.06 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 555 Alkyne is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AFDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AFDye™ 555 Alkyne can be used a less expensive alternative to Alexa Fluor® 555 Alkyne.

For application where the presence of copper is not acceptable, please consider our AFDye™ 555 DBCO or Cy3 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye555

Related Products
AFDye 647 DBCO

Excitation maximum: 648 nm
Emission maximum: 671 nm

AFDye 594 DBCO

Excitation maximum: 590 nm
Emission maximum: 617 nm