AFDye 546 Tyramide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1539-1
150 slides
$149.00
In stock
1539-2
1500 slides
$875.00
In stock

    Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

    AFDye™ 546 Tyramide (Alexa Fluor® 546 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

    Abs/Em Maxima
    543/563 nm
    Extinction Coefficient
    105,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    543 or 546 nm
    Spectrally Similar Dyes
    Alexa Fluor® 546, Atto™ 543, CF® 543
    Molecular weight
    939.08
    CAS
    N/A
    Solubility
    Water, DMSO, DMF, MeOH
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    MB543

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