AZDye 546 Picolyl Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1284-1
1 mg
$179.00
In stock
1284-5
5 mg
$549.00
In stock
1284-25
25 mg
$1,535.00
In stock

    AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    Learn more about picolyl azide reagents.
    Abs/Em Maxima
    554/570 nm
    Extinction Coefficient
    110,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    543 nm or 546 nm
    Spectrally Similar Dyes
    Alexa Fluor® 546, Atto™ 546, CF® 546
    Molecular weight
    1050.39 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

    In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

    In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

    AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

    AZDye546

    1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
    2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
    3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
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