AFDye 546 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1286-1
1 mg
$139.00
In stock
1286-5
5 mg
$445.00
In stock
1286-25
25 mg
$1,395.00
In stock

AFDye™ 546 DBCO (Alexa Fluor® 546 DBCO equivalent) is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
554/570 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Spectrally Similar Dyes
Alexa Fluor® 546, Atto™ 546, CF® 546
Molecular weight
1006.50
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 546 DBCO (Alexa Fluor® 546 DBCO equivalent) is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AFDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye546

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