AZDye 546 DBCO
AZDye™ 546 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 546 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AZDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AZDye™ 546 dye is spectrally almost identical Alexa Fluor® 546, CF 543 and TAMRA.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Excitation maximum: 648 nm
Emission maximum: 671 nm
Excitation maximum: 590 nm
Emission maximum: 617 nm