AFDye 546 Azide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1283-1
1 mg
$139.00
In stock
1283-5
5 mg
$395.00
In stock
1283-25
25 mg
$1,295.00
In stock

AFDye™ 546 Azide is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
554/570 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Spectrally Similar Dyes
Alexa Fluor® 546, Atto™ 546, CF® 546
Molecular weight
972.36 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 546 Azide is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AFDye™ 546 (Alexa Fluor® 546 equivalent) is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye546

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