AFDye 546 Alkyne
AFDye™ 546 Alkyne (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 546 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
A probe for copper-less azide detection (AFDye™ 546 DBCO or MB 543 DBCO) is also available for application where the presence of copper is not acceptable.
Alexa Fluor® is a registered trademark of ThermoFisher Scientific.
AFDye™ 546 Alkyne (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AFDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AFDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.
For application where the presence of copper is not acceptable, please consider our AFDye™ 546 DBCO or MB 543 DBCO probes for copper-less detection of azide-modified molecules.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Excitation maximum: 590 nm
Emission maximum: 617 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm