AFDye 546 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1285-1
1 mg
$139.00
In stock
1285-5
5 mg
$395.00
In stock
1285-25
25 mg
$1,295.00
In stock

AFDye™ 546 Alkyne (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 546 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AFDye™ 546 DBCO or MB 543 DBCO) is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of ThermoFisher Scientific.

Abs/Em Maxima
554/570 nm
Extinction Coefficient
110,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Spectrally Similar Dyes
Alexa Fluor® 546, Atto™ 546, CF® 546
Molecular weight
855.34 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 546 Alkyne (Alexa Fluor® 546 Azide equivalent) is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AFDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AFDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.

For application where the presence of copper is not acceptable, please consider our AFDye™ 546 DBCO or MB 543 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AFDye546

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