AFDye 532 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1339-1
1 mg
$139.00
In stock
1339-5
5 mg
$395.00
In stock
1339-25
25 mg
$1,295.00
In stock
1339-100
100 mg
$2,695.00
In stock

AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
532/554 nm
Extinction Coefficient
78,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Spectrally Similar Dyes
Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight
723.77 (protonated)
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AFDye™ 532 is a water-soluble, highly photostable, and bright yellow dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AFDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

Alexa Fluor® 532 Dye is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye532

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