AFDye 532 NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
1339-1
1 mg
$139.00
In stock
1339-5
5 mg
$395.00
In stock
1339-25
25 mg
$1,295.00
In stock
1339-100
100 mg
$2,695.00
In stock

    AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

    Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    532/554 nm
    Extinction Coefficient
    78,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    532 nm
    Spectrally Similar Dyes
    Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
    Molecular weight
    723.77 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 532 NHS Ester (Alexa Fluor® 532 NHS Ester equivalent) is an amine reactive, yellow emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

    AFDye™ 532 is a water-soluble, highly photostable, and bright yellow dye with pH insensitive emission from pH 4 to pH 10. The excitation of AFDye™ 532 ideally suited for the frequency-doubled Nd:YAG laser line. AFDye™ 532 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

    AFDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

    Alexa Fluor® 532 Dye is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AFDye532

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