AZDye 532 Maleimide
AZDye™ 532 Maleimide is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.
Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to either a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.
AZDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF® 532 Dye.
Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.
Excitation maximum: 494 nm
Emission maximum: 517 nm
Excitation maximum: 648 nm
Emission maximum: 671 nm
Excitation maximum: 590 nm
Emission maximum: 617 nm