AZDye 532 Maleimide

Catalog#
Unit Size
Price (USD)
Availability
Qty
1521-1
1 mg
$159.00
In stock
1521-5
5 mg
$495.00
In stock
1521-25
25 mg
$1,495.00
In stock
1521-100
100 mg
$2,795.00
In stock

    AZDye™ 532 Maleimide is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

    Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to either a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

    AZDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF® 532 Dye.

    Alexa Fluor® Dyes is a registered trademark of Thermo Fisher Scientific.

    Abs/Em Maxima
    532/554 nm
    Extinction Coefficient
    78,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    532 nm
    Spectrally Similar Dyes
    Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
    Molecular weight
    790.90 (protonated)
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AZDye532

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