AFDye 532 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1282-1
1 mg
$139.00
In stock
1282-5
5 mg
$445.00
In stock
1282-25
25 mg
$1,395.00
In stock

AFDye™ 532 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Maxima
532/554 nm
Extinction Coefficient
78,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Spectrally Similar Dyes
Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight
878.96
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 532 DBCO an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AFDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AFDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

Alexa Fluor® 532 Dye is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye532

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