AFDye 532 DBCO

Catalog#
Unit Size
Price (USD)
Availability
Qty
1282-1
1 mg
$149.00
In stock
1282-5
5 mg
$475.00
In stock
1282-25
25 mg
$1,495.00
In stock

    AFDye™ 532 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    Abs/Em Maxima
    532/554 nm
    Extinction Coefficient
    78,000
    Flow Cytometry Laser Line
    532 nm
    Microscopy Laser Line
    532 nm
    Spectrally Similar Dyes
    Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
    Molecular weight
    878.96
    CAS
    N/A
    Solubility
    Water, DMSO, DMF
    Purity
    >95% (HPLC)
    Appearance
    Orange to light red solid
    Storage conditions
    -20°C. Desiccate
    Shipping conditions
    Ambient temperature

    AFDye™ 532 DBCO an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AFDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

    AFDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

    AFDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

    Alexa Fluor® 532 Dye is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

    AFDye532

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