AFDye 532 Alkyne

Catalog#
Unit Size
Price (USD)
Availability
Qty
1281-1
1 mg
$139.00
In stock
1281-5
5 mg
$395.00
In stock
1281-25
25 mg
$1,295.00
In stock

AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC). AFDye™ 532 Alkyne is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye 532 dyes are well suited to direct imaging of low-abundance targets.

A probe for copper-free azide detection is also available for application where the presence of copper is not acceptable.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Abs/Em Maxima
532/554 nm
Extinction Coefficient
78,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Spectrally Similar Dyes
Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight
651.61
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Orange to light red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature

AFDye™ 532 Alkyne (Alexa Fluor® 532 Alkyne equivalent) is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC).

AFDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

The absorption/emission spectra of AFDye™ 532 is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

For application where the presence of copper is not acceptable, please consider our AFDye™ 532 DBCO probe for copper-less detection of azide-modified molecules.

Alexa Fluor® 532 Dye is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AFDye532

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